Castor bean lipase: action on its endogenous substrate
نویسندگان
چکیده
منابع مشابه
Lipase Activities in Castor Bean Endosperm during Germination.
Two lipases were found in extracts from castor bean (Ricinus communis L.) endosperm. One, with optimal activity at pH 5.0 (acid lipase), was present in dry seeds and displayed high activity during the first 2 days of germination. The second, with an alkaline pH optimum (alkaline lipase), was particularly active during days 3 to 5. When total homogenates of endosperm were fractionated into fat l...
متن کاملLectins in castor bean seedlings.
The amounts of the two lectins (ricin and Ricinus communis agglutinin) in tissues of castor bean seedlings were followed during germination and early growth. For measurement, lectins in extracts were separately eluted from Sepharose columns; an antibody to the agglutinin was also used to detect the lectins by immunodiffusion. The endosperm of the dry seed contains 3.5 mg total lectin (5.6% of t...
متن کاملPhosphatidylcholine synthesis in castor bean endosperm.
Three pathways for phosphatidylcholine synthesis were assayed in castor bean (Ricinus communis var. Hale) endosperm. Phosphatidylethanolamine: S-adenosylmethionine methyl transferase occurred predominantly in the endoplasmic reticulum fraction, but some activity appeared in the mitochondria. Phosphorylcholine glyceride transferase occurred exclusively in the endoplasmic reticulum. The phosphory...
متن کاملPyruvate Dehydrogenase Complex from Germinating Castor Bean
Subceliular organelles from castor bean (Ricuns commnwuis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postbit o. Marker enzyme activities of the organeUes were measured (fumarase, catalase, and triose phosphate isomerase) and the homogeneity of the organelle fractions was examined by electron microscopy. Pyrumate dehydrogenase comple...
متن کاملPhosphatidylethanolamine synthesis in castor bean endosperm.
Phosphatidylethanolamine synthesis by CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) from the endoplasmic reticulum of castor bean (Ricinus communis L. var. Hale) endosperm was characterized. The Michaelis-Menten constant of the enzyme for CDP-ethanolamine was approximately 8.0 micromolar. The pH optimum was 6.5 and a divalent cation was an absolute requirement ...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1960
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39064-7